| 质粒名称: | pOpen-MMLV_RT (mut H) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 165546 |
| 存储实验室: | Drew Endy, Jennifer Molloy, FreeGenes Project |
|
载体骨架基本信息 |
|
| 载体名称: | pBuild |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 4109 bp |
| 载体修饰: | -- |
| 载体类型: | Synthetic Biology |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 莫罗尼小鼠白血病病毒（MMLV）逆转录酶（RNAse H被3个突变失活） |
| 物种来源: | Other |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AATG(未知是否破坏) |
| 3’克隆位点: | GCTT (未知是否破坏) |
| 5’测序引物 | M13 forward |
| 3’测序引物 | M13 reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pOpen-MMLV_RT (mut H) was a gift from Drew Endy & Jennifer Molloy & FreeGenes Project (Addgene plasmid # 165546 ; http://n2t.net/addgene:165546 ; RRID:Addgene_165546) |
| 参考文献部分: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pOpen-MMLV_RT (mut H) 4109 bp ds-DNA circular SYN 10-AUG-2021
DEFINITION Single 75 kDa monomer, cDNA synthesis; high enzyme activity and
processivity. .
ACCESSION .
VERSION .
KEYWORDS pOpen-MMLV_RT (mut H)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4109)
TITLE Open Enzyme Collection
REFERENCE 2 (bases 1 to 4109)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 10, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4109
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2132..2150)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 2146..2193
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(2198..2214)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2198..2214)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
rep_origin complement(2281..2869)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2361..2380)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3060..3920)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3683..3702
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3921..4025)
/gene="bla"
/label=AmpR promoter
primer_bind 4041..4057
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator 4058..4089
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
ORIGIN
1 aaaggggccg tcaatatcag ggtctctaat gactctgaac atcgaagatg aacatcgtct
61 gcacgaaacc agcaaagaac cggacgtttc tctgggttca acctggctgt ctgattttcc
121 gcaagcttgg gccgaaactg gcggtatggg cctggccgtc cgtcaggcac cgctgatcat
181 cccactgaaa gcgacctcca ccccggtgtc catcaaacag tatccaatgt cccaggaagc
241 acgtctgggc atcaagccac atatccaacg tctgctggat caaggtatcc tggtaccgtg
301 tcaatcgcct tggaacactc cactgctgcc ggtgaaaaaa ccgggcacta acgattaccg
361 tccggtacag gacctgcgtg aagttaacaa acgtgttgaa gatattcatc cgaccgtccc
421 gaatccgtac aacctcctgt ccggtctgcc gccgagccat cagtggtaca ccgtgctgga
481 cctgaaggac gcattctttt gcctgcgtct gcacccgacc tctcagccgc tgtttgcgtt
541 tgaatggcgt gacccggaaa tgggtatttc tggccagctt acatggaccc gcctgccaca
601 gggtttcaaa aactccccga cccttttcga cgaagccctg caccgtgacc tggcagattt
661 tcgcattcag cacccggacc tcatcctgct gcaatacgtg gacgatctgc tgttggcagc
721 taccagcgaa ctggattgcc agcagggtac ccgtgcgctg ctgcaaaccc tgggcaacct
781 gggttaccgt gcttccgcga aaaaggcgca gatttgccaa aaacaggtca aatatctggg
841 ttacctgctg aaagaaggcc agcgctggct gaccgaggcc cgcaaagaaa ccgtgatggg
901 tcaaccgacc ccgaaaaccc cgcgtcaact gcgcgagttt ctgggtaccg ctggcttctg
961 ccgtctgtgg attccgggct tcgctgaaat ggccgcgccg ctgtatccgt taaccaaaac
1021 tggtactctg ttcaactggg gtccggacca gcagaaagcc tatcaggaga ttaaacaagc
1081 gctgctgacc gcaccggcgc tgggcctgcc ggacctgacc aaaccatttg aactgttcgt
1141 tgacgagaaa caaggttacg ctaaaggtgt tcttacccag aaactgggtc cgtggcgccg
1201 tccggtagcg tatctgtcta aaaaactgga cccggttgcg gctggttggc cgccgtgtct
1261 gcgtatggtt gctgctattg ccgtactgac taaggatgcg ggcaaactga ctatgggcca
1321 gccgctggtg atcctggctc cgcatgcggt ggaggccctg gttaaacagc cgccggatcg
1381 ctggctgtct aacgcgcgta tgacacacta ccaggctctg ctgctggata ccgaccgtgt
1441 tcagttcggc ccggtcgtgg ccctgaaccc ggcgaccctg ctcccgctgc cggaagaagg
1501 tctgcaacac aattgcctgg atattctggc ggaagcccat ggtactcgtc cggatctgac
1561 cgaccagcct ctgccggatg cagaccacac ctggtacact ggtggcagct ccctcctgca
1621 agagggccag cgtaaggcag gcgcggctgt taccacggaa accgaagtaa tctgggctaa
1681 agccctgcca gcaggaacgt cagcccaacg cgcacaactg attgctctga cccaggcact
1741 gaaaatggcg gaaggtaaaa agctgaacgt ttacactaac tcccgctacg cattcgctac
1801 cgcccacatc catggcgaaa tctacagacg tcgtggtctg ctgacctctg aaggcaaaga
1861 aatcaaaaac aaagatgaga tcctggccct gctgaaagcg ctgttcctgc ctaaacgttt
1921 atcaatcatc cactgtccgg gccaccagaa gggccactcc gcggaagcgc gcggcaaccg
1981 tatggcagac caggcagcac gcaaagctgc ctgaagagct ttgagaccag tatctcagca
2041 agggcaaccg acgttgtgag agcgcggact agggataaca gggtaatgct tccatcagtg
2101 agaagaggcg ctgaggtgtc aatcgtcgga gccgctgagc aataactagc ataacccctt
2161 ggggcctcta aacgggtctt gaggggtttt ttgcatggtc atagctgttt cctgagagct
2221 tggcaggtga tgacacacat taacaaattt cgtgaggagt ctccagaaga atgccattaa
2281 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
2341 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
2401 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
2461 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
2521 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
2581 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
2641 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
2701 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
2761 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
2821 tttttttgtt tgcaagcagc agattacacg cagaaaaaaa ggatctcaag aaggcctact
2881 attagcaaca acgatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
2941 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
3001 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
3061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
3121 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
3181 agtgctgcaa tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac
3241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
3301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
3361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
3421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
3481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
3541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
3601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
3661 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
3721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
3781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
3841 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
3901 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
3961 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
4021 ccgcgcacct gcaccagtca gtaaaacgac ggccagtagt caaaagcctc cgaccggagg
4081 cttttgactt ggttcaggtg gagtgggag
//
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